Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 61, Issue 2, Pages 116-124Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155412470455
Keywords
tissue microarray; nucleic acids; IHC; cancer markers; cell type specific expression
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Funding
- Regione Lombardia Metadistretto
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Tissue microarray (TMA) and cell microarray (CMA) are two powerful techniques that allow for the immunophenotypical characterization of hundreds of samples simultaneously. In particular, the CMA approach is particularly useful for immunophenotyping new stem cell lines (e. g., cardiac, neural, mesenchymal) using conventional markers, as well as for testing the specificity and the efficacy of newly developed antibodies. We propose the use of a tissue arrayer not only to perform protein expression profiling by immunohistochemistry but also to carry out molecular genetics studies. In fact, starting with several tissues or cell lines, it is possible to obtain the complete signature of each sample, describing the protein, mRNA and microRNA expression, and DNA mutations, or eventually to analyze the epigenetic processes that control protein regulation. Here we show the results obtained using the Galileo CK4500 TMA platform. (J Histochem Cytochem 61: 116-124, 2013)
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