Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 61, Issue 2, Pages 156-168Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155412466872
Keywords
IA-2; islet cell antigen ICA512; IA-2 beta; phogrin; protein tyrosine phosphatases; gastrointestinal endocrine cell; secretory granules; gut hormones
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Funding
- Japan Society for the Promotion of Science (JSPS) [22500367, 21689007]
- Institute for Molecular and Cellular Regulation, Gunma University
- Grants-in-Aid for Scientific Research [22500367, 25450471, 21689007, 24390050] Funding Source: KAKEN
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Islet-associated protein-2 (IA-2) and IA-2 beta (also known as phogrin) are unique neuroendocrine-specific protein tyrosine phosphatases (PTPs). The IA-2 family of PTPs was originally identified from insulinoma cells and discovered to be major autoantigens in type 1 diabetes. Despite its expression in the neural and canonical endocrine tissues, data on expression of the IA-2 family of PTPs in gastrointestinal endocrine cells (GECs) are limited. Therefore, we immunohistochemically investigated the expression of the IA-2 family of PTPs in the rat gastrointestinal tract. In the stomach, IA-2 and IA-2 beta were expressed in GECs that secrete serotonin, somatostatin, and cholecystokinin/gastrin-1. In addition to these hormones, secretin, gastric inhibitory polypeptide (also known as the glucose-dependent insulinotropic peptide), glucagon-like peptide-1, and glucagon, but not ghrelin were coexpressed with IA-2 or IA-2 beta in duodenal GECs. Pancreatic islet cells that secrete gut hormones expressed the IA-2 family of PTPs. The expression patterns of IA-2 and IA-2 beta were comparable. These results reveal that the IA-2 family of PTPs is expressed in a cell type-specific manner in rat GECs. The extensive expression of the IA-2 family of PTPs in pancreo-gastrointestinal endocrine cells and in the enteric plexus suggests their systemic contribution to nutritional control through a neuroendocrine signaling network. (J Histochem Cytochem 61: 156-168, 2013)
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