Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 58, Issue 4, Pages 369-376Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/jhc.2009.954909
Keywords
islet cells; beta-cells; immunohistochemistry
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Funding
- National Institutes of Health [DK-44523, DK-66056, DK-61251]
- Joslin Diabetes Endocrine Research Center [DK-36836]
- Juvenile Diabetes Research Foundation [JDRF 1-2004-120, 1-2000-389]
- Endocrine Fellow Foundation award
- Lawson-Wilkins Fellowship
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Markers of beta-cell maturity would be useful in staging the differentiation of stem/progenitor cells to beta-cells whether in vivo or in vitro. We previously identified markers for newly formed beta-cells in regenerating rat pancreases after 90% partial pancreatectomy. To test the generality of these markers of newly formed beta-cells, we examined their expression during the perinatal period, a time of recognized beta-cell immaturity. We show by semiquantitative RT-PCR and immunostaining over the time course from embryonic day 18/20 to birth, 1 day, 2 days, 3 days, 7 days, and adult that MMP-2, CK-19, and SPD are truly markers of new and immature beta-cells and that their expression transiently peaks in the perinatal period and is not entirely synchronous. The shared expression of these markers among fetal, newborn, and newly regenerated beta-cells, but not adult, strongly supports their use as potential markers for new beta-cells in the assessment of both the maturity of stem cell derived insulin-producing cells and the presence of newly formed islets (neogenesis) in the adult pancreas. (J Histochem Cytochem 58:369-376, 2010)
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