Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 57, Issue 5, Pages 503-512Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/jhc.2009.952929
Keywords
eukaryotic translation initiation factor 2 alpha; eukaryotic translation initiation factor 4E; cyclin D1; brain tumors; translation
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Funding
- Fundacion Mutua Madrilena (Madrid, Spain) [FMM06/180]
- Fundacion para la Investigacion Biomedica del Hospital Universitario Ramon y Cajal
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Increased protein synthesis is regulated, in part, by two eukaryotic translation initiation factors (eIFs): eIF4E and eIF2 alpha. One or both of these factors are often overexpressed in several types of cancer cells; however, no data are available at present regarding eIF4E and eIF2 alpha levels in brain tumors. in this study, we analyzed the expression, subcellular localization and phosphorylation states of eIF4E and eIF2 alpha in 64 brain tumors (26 meningiomas, 16 oligodendroglial tumors, and 22 astrocytomas) and investigated the correlation with the expression of MIB-1, p53, and cyclin D1 proteins as well. There are significant differences in the phosphorylated eIF4E levels between the tumors studied, being the highest in meningiomas and the lowest in the oligodendroglial tumors. Relative to subcellular localization, eIF4E is frequently found in the nucleus of the oligodendroglial tumors and rarely in the same compartment of the meningiomas, whereas eIF2 alpha showed an inverse pattern. Finally, cyclin D1 levels directly correlate with the phosphorylation status of both factors. The different expression, phosphorylation, or/and subcellular distribution of eIF2 alpha and eIF4E within the brain types of tumors studied could indicate that different pathways are activated for promoting cell cycle proliferation, for instance, leading to increased cyclin D1 expression. (J Histochem Cytochem 57:503-512, 2009)
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