Journal
JOURNAL OF HEPATOLOGY
Volume 50, Issue 1, Pages 42-48Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhep.2008.08.020
Keywords
Hepatitis B virus; Lamivudine; Adefovir; Entecavir; Antiviral drug-resistant mutations
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Funding
- Ufuk University
- Bristol-Myers Squibb Foundation Virology Fellowship
- Tuktawa Foundation for Liver Research and Education
- GlaxoSmithKline
- Gilead
- Bristol-Myers Squibb
- Idenix/Novartis
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Background/Aims: Early detection of antiviral drug-resistant mutations enables prompt initiation of rescue therapy. The aim of this study was to determine the accuracy and sensitivity of a new line probe assay in the detection of antiviral drug-resistant HBV mutations. Methods: One-hundred samples from 54 patients with virologic breakthrough during entecavir, lamivudine or adefovir treatment and 21 samples from 21 nucleoside-naive patients were tested by direct sequencing and an updated line probe assay (Innogenetics, HBV DR v.3) which incorporates probes that can detect mutations at 11 positions of the reverse transcriptase region of the HBV polymerase gene. Results: Complete concordance between line probe and sequencing results was observed for 90/121 samples (74.3%) and 1291/1331 amino acid positions (96.9%). Testing of follow-up samples and clonal analysis of discordant samples confirmed the presence of mutations where line probe assay but not direct sequencing detected mutations. HBV DR v.3 assay consistently detected mutations present in >= 5% of the virus population when HBV DNA concentration was >= 4 log(10) copies/mL. Conclusions: The updated version of the line probe assay (HBV DR v.3) has high concordance with direct sequencing in detecting antiviral drug-resistant mutations but its sensitivity in detecting mutations at some positions needs to be improved. (C) 2008 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
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