Journal
JOURNAL OF HELMINTHOLOGY
Volume 87, Issue 2, Pages 189-194Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0022149X12000181
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Funding
- Fundacao para a Ciencia e a Tecnologia (FCT) [PTDC/BIA-BDE/74349/2006, SFRH/BPD/26546/2006, SFRH/BD/74305/2010]
- Spanish Herpetological Society
- Spanish Ministry of Environment
- Fundação para a Ciência e a Tecnologia [SFRH/BD/74305/2010, PTDC/BIA-BDE/74349/2006] Funding Source: FCT
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The development of molecular methods is becoming a promising field in the analysis of parasite fauna in wildlife species. This is especially useful in the case of parasite species where developmental larval stages are difficult to assess using standard methods. In this study we screened for the presence of parasitic nematodes infecting lacertid lizards from the Iberian Peninsula and Balearic islands using nematode-specific 18S ribosomal RNA gene primers on host tissue samples. Sequencing of positive samples revealed the presence of different genera of nematodes. The detection of Strongyloides, a monoxenous genus reported for the first time in Podarcis lilfordi is probably the result of the amplification of larval stages present in the host circulatory system. Two spirurid nematodes, Synhimantus and a new unidentified clade, were also found, suggesting that reptiles might be paratenic hosts of several spirurid species. This study demonstrates the benefits of using specific molecular markers on tissue samples to identify infecting larval stages of nematodes, otherwise difficult to assess using traditional screening methods.
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