Journal
JOURNAL OF HEART AND LUNG TRANSPLANTATION
Volume 38, Issue 1, Pages 92-99Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.healun.2018.09.025
Keywords
cold ischemia time; heart transplantation; ischemia-reperfusion; mitochondria; mitochondrial transplantation
Funding
- Boston Children's Hospital Anesthesia Foundation
- Ryan Family Endowment
- Cardiac Conduction Fund
- National Institutes of Health [5T32HL007734]
- Richard A. and Susan F. Smith President's Innovation Award
- Sidman Family Foundation
- Michael B. Rukin Charitable Foundation
- Kenneth C. Griffin Charitable Research Fund
- Boston Investment Council
- Michael B. Klein and Family
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL108107, T32HL007734] Funding Source: NIH RePORTER
Ask authors/readers for more resources
BACKGROUND: Cold ischemia time (CIT) causes ischemia-reperfusion injury to the mitochondria and detrimentally effects myocardial function and tissue viability. Mitochondrial transplantation replaces damaged mitochondria and enhances myocardial function and tissue viability. Herein we investigated the efficacy of mitochondrial transplantation in enhancing graft function and viability after prolonged CIT. METHODS: Heterotopic heart transplantation was performed in C57BL/6J mice. Upon heart harvesting from C57BL/6J donors, 0.5 ml of either mitochondria (1 x 10(8) in respiration buffer; mitochondria group) or respiration buffer (vehicle group) was delivered antegrade to the coronary arteries via injection to the coronary ostium. The hearts were excised and preserved for 29 +/- 0.3 hours in cold saline (4 degrees C). The hearts were then heterotopically transplanted. A second injection of either mitochondria (1 x 10(8)) or respiration buffer (vehicle) was delivered antegrade to the coronary arteries 5 minutes after transplantation. Grafts were analyzed for 24 hours. Beating score, graft function, and tissue injury were measured. RESULTS: Beating score, calculated ejection fraction, and shortening fraction were significantly enhanced (p < 0.05), whereas necrosis and neutrophil infiltration were significantly decreased (p < 0.05) in the mitochondria group as compared with the vehicle group at 24 hours of reperfusion. Transmission electron microscopy showed the presence of contraction bands in vehicle but not in mitochondria grafts. CONCLUSIONS: Mitochondrial transplantation prolongs CIT to 29 hours in the murine heart transplantation model, significantly enhances graft function, and decreases graft tissue injury. Mitochondrial transplantation may provide a means to reduce graft failure and improve transplantation outcomes after prolonged CIT. (C) 2018 International Society for Heart and Lung Transplantation. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available