Journal
OPTICS LETTERS
Volume 40, Issue 18, Pages 4305-4308Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.40.004305
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Funding
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/I022074/1, BB/I022937/1]
- Engineering and Physical Sciences Research Council (EPSRC) [EP/K03197X/1]
- Medical Research Council (MRC) [MR/K015664/1]
- ST Microelectronics, Imaging Division, Edinburgh
- Biotechnology and Biological Sciences Research Council [BB/I022937/1, BB/I022074/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/M01326X/1, EP/K03197X/1] Funding Source: researchfish
- Medical Research Council [MR/K015664/1] Funding Source: researchfish
- BBSRC [BB/I022074/1, BB/I022937/1] Funding Source: UKRI
- EPSRC [EP/M01326X/1, EP/K03197X/1] Funding Source: UKRI
- MRC [MR/K015664/1] Funding Source: UKRI
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We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) events from a 32 x 32 complementary metal oxide semiconductor (CMOS) single photon avalanche detector (SPAD) array (Megaframe) to the computer memory. Custom firmware was written to transmit event codes from 1024-TCSPC-enabled pixels for fast transfer of TCSPC events. Our 1024-channel TCSPC system is capable of acquiring up to 0.5 x 10(9) TCSPC events per second with 16 histogram bins spanning a 14 ns width. Other options include 320 x 10(6) TCSPC events per second with 256 histogram bins spanning either a 14 or 56 ns time window. We present a wide-field fluorescence microscopy setup demonstrating fast fluorescence lifetime data acquisition. To the best of our knowledge, this is the fastest direct TCSPC transfer from a single photon counting device to the computer to date. (C) 2015 Optical Society of America
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