Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 199, Issue -, Pages 58-63Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2011.10.053
Keywords
N,N-Dimethylformamide; Immobilization; Alginate; Polyvinyl alcohol; PVA-alginate blend; Ochrobactrum sp.
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Funding
- Department of Biotechnology (DBT), Ministry of Science and Technology, New Delhi, India
- University Grants Commission, New Delhi
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Effective removal of dimethylformamide (DMF), the organic solvent found in industrial effluents of textile and pharma industries, was demonstrated by using free and immobilized cells of Ochrobactrum sp. DGVK1, a soil isolate capable of utilizing DMF as a sole source of carbon, nitrogen. The free cells have efficiently removed DMF from culture media and effluents, only when DMF concentration was less than 1% (v/v). Entrapment of cells either in alginate or in polyvinyl alcohol (PVA) failed to increase tolerance limits. However, the cells of Ochrobactrum sp. DGVK1 entrapped in PVA-alginate mixed matrix tolerated higher concentration of DMF (2.5%, v/v) and effectively removed DMF from industrial effluents. As determined through batch fermentation, these immobilized cells have retained viability and degradability for more than 20 cycles. A continuous packed bed reactor, generated by using PVA-alginate beads, efficiently removed DMF from industrial effluents, even in the presence of certain organic solvents frequently found in effluents along with DMF. (C) 2011 Elsevier B.V. All rights reserved.
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