Lipopolysaccharide-Treated Human Gingival Fibroblasts Continuously Produce PGE2

The aim of this study was to examine whether human gingival fibroblasts (HGFs) sustain to produce prostaglandin E-2 (PGE(2)) in the presence of lipopolysaccharide (LPS). HGFs were treated with or without 10 ng/ml of LPS from Porphyromonas gingivalis (PgLPS) for 7 days collecting the culture supernatants for every 24 h, and PGE(2) concentration in the supernatants was measured by ELISA. HGFs produced PGE(2) at low level without PgLPS treatment. In contrast, HGFs continuously produce PGE(2) with PgLPS treatment. At each day, the amount of PGE(2) production by HGFs with PgLPS treatment was higher than that without treatment. These results demonstrate that the characteristic of HGFs, which continuously produce PGE(2) in the presence of LPS, sustains inflammatory response in periodontal disease.