Journal
OPTICS EXPRESS
Volume 23, Issue 21, Pages 26999-27010Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.23.026999
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Funding
- National Basic Research Program of China (973 Program) [2015CB352003]
- NIH [9P41EB015871-26A1, 1R01HL121386-01A1]
- Hamamatsu Corp
- Singapore-MIT Alliance for Science and Technology Center (BioSym IRG)
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We report a novel approach to Fourier ptychographic microscopy (FPM) by using a digital micromirror device (DMD) and a coherent laser source (532 nm) for generating spatially modulated sample illumination. Previously demonstrated FPM systems are all based on partially-coherent illumination, which offers limited throughput due to insufficient brightness. Our FPM employs a high power coherent laser source to enable shot-noise limited high-speed imaging. For the first time, a digital micromirror device (DMD), imaged onto the back focal plane of the illumination objective, is used to generate spatially modulated sample illumination field for ptychography. By coding the on/off states of the micromirrors, the illumination plane wave angle can be varied at speeds more than 4 kHz. A set of intensity images, resulting from different oblique illuminations, are used to numerically reconstruct one high-resolution image without obvious laser speckle. Experiments were conducted using a USAF resolution target and a fiber sample, demonstrating high-resolution imaging capability of our system. We envision that our approach, if combined with a coded-aperture compressive-sensing algorithm, will further improve the imaging speed in DMD-based FPM systems. (C) 2015 Optical Society of America
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