Journal
JOURNAL OF GENERAL VIROLOGY
Volume 95, Issue -, Pages 2462-2467Publisher
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.068023-0
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Funding
- European Union [278433]
- European Virus Archive (European Union Seventh Framework Programme) [228292]
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Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified infectious RNA viruses were generated in days using the newly described ISA (infectious-subgenomic-amplicons) method. This new versatile and simple procedure may enhance our capacity to obtain infectious RNA viruses from PCR-amplified genetic material.
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