Journal
JOURNAL OF GENERAL VIROLOGY
Volume 95, Issue -, Pages 2071-2074Publisher
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.066019-0
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Funding
- National Institutes of Health [HHSN272201000040I/HHSN27200004/D04]
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The genus Negevirus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed in vitro and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.
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