Journal
JOURNAL OF GENERAL VIROLOGY
Volume 96, Issue -, Pages 513-523Publisher
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.000005
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Funding
- Wellcome Trust [099220]
- Medical Research Council
- FAPESP-Sao Paulo Research Foundation (CNPQ) [2013/02798-0]
- CAPES-National Council for the Improvement of Higher Education (Brazil) scholarship [3851/10-9]
- Medical Research Council [1101085] Funding Source: researchfish
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Oropouche virus (OROV) is a medically important orthobunyavirus, which causes frequent outbreaks of a febrile illness in the northern parts of Brazil. However, despite being the cause of an estimated half a million human infections since its first isolation in Trinidad in 1955, details of the molecular biology of this tripartite, negative-sense RNA virus remain limited. We have determined the complete nucleotide sequence of the Brazilian prototype strain of OROV, BeAn 19991, and found a number of differences compared with sequences in the database. Most notable were that the S segment contained an additional 204 nt at the 3' end and that there was a critical nucleotide mismatch at position 9 within the base-paired terminal panhandle structure of each genome segment. In addition, we obtained the complete sequence of the Trinidadian prototype strain TRVL-9760 that showed similar characteristics to the BeAn 19991 strain. By using a 17 RNA polymerase-driven minigenome system, we demonstrated that cDNA clones of the BeAn 19991 L and S segments expressed functional proteins, and also that the newly determined terminal untranslated sequences acted as functional promoters in the minigenome assay. By co-transfecting a cDNA to the viral glycoproteins, virus-like particles were generated that packaged a minigenome and were capable of infecting naive cells.
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