Journal
JOURNAL OF GENERAL VIROLOGY
Volume 94, Issue -, Pages 1734-1738Publisher
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.053025-0
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Funding
- Medical Research Council
- Nuffield Foundation
- Medical Research Council [MC_UP_A550_1029, MC_UU_12014/7] Funding Source: researchfish
- MRC [MC_UP_A550_1029, MC_UU_12014/7] Funding Source: UKRI
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Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. We have previously described the X-ray structure of an NC-like structure: a decameric ring formed of N-RNA that mimics one turn of the helical NC. In the absence of experimental data we had hypothesized that the NC helix would be right-handed, as the N-N contacts in the ring appeared to more-easily-adapt to-that conformation We-now unambiguously show that the RSV NC is a left-handed helix. We further show that the Contacts in the ring can be distorted to Maintain key N-N-protein interactions in a left-handed helix, and discuss the implications of the resulting atomic model of the helical NC for viral replication and transcription.
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