Journal
JOURNAL OF GENERAL VIROLOGY
Volume 94, Issue -, Pages 1576-1586Publisher
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.049635-0
Keywords
-
Categories
Funding
- Wellcome Trust
- BBSRC
- EU-FP7 LipidomicNet project
- Biomedical Research Centre
- BBSRC [BBS/E/B/000C0415, BBS/E/B/0000S227] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/B/000C0415, BBS/E/B/0000S227] Funding Source: researchfish
Ask authors/readers for more resources
Rotaviruses (RVs) cause acute gastroenteritis in infants and young children, and are globally distributed. Within the infected host cell, RVs establish replication complexes in viroplasms ('viral factories') to which lipid droplet organelles are recruited. To further understand this recently discovered phenomenon, the lipidomes of RV-infected and uninfected MA104 cells were investigated. Cell lysates were subjected to equilibrium ultracentrifugation through iodixanol gradients. Fourteen different classes of lipids were differentiated by mass spectrometry. The concentrations of virtually all lipids were elevated in RV-infected cells. Fractions of low density (1.11-1.15 g ml(-1)), in which peaks of the RV dsRNA genome and lipid droplet- and viroplasm-associated proteins were observed, contained increased amounts of lipids typically found concentrated in the cellular organelle lipid droplets, confirming the close interaction of lipid droplets with viroplasms. A decrease in the ratio of the amounts of surface to internal components of lipid droplets upon RV infection suggested that the lipid droplet-viroplasm complexes became enlarged.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available