Journal
JOURNAL OF GENERAL VIROLOGY
Volume 92, Issue -, Pages 2542-2548Publisher
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.034983-0
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Funding
- Netherlands Organization for Scientific Research (NWO-CW) [700-57-301]
- EU [260644]
- Swiss National Science Foundation
- NIH [AI72493]
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Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.
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