Journal
JOURNAL OF GENERAL VIROLOGY
Volume 90, Issue -, Pages 527-535Publisher
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.007674-0
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Funding
- United States Department of Agriculture [USDA 2006-01616]
- South Dakota Animal Disease Research and Diagnostic Laboratory
- South Dakota Agricultural Experiment Station
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Porcine reproductive and respiratory virus (PRRSV) is an enveloped positive-sense RNA virus of the family Arteriviridae that causes severe and persistent disease in pigs worldwide. The PRRSV virion consists of a lipid envelope that contains several envelope proteins surrounding a nucleocapsid core that encapsidates the RNA genome. To provide a better understanding of the structure and assembly of PRRSV, we have carried out cryo-electron microscopy and tomographic reconstruction of virions grown in MARC-145 cells. The virions are pleomorphic, round to egg-shaped particles with an average diameter of 58 nm. The particles display a smooth outer surface with only a few protruding features, presumably corresponding to the envelope protein complexes. The virions contain a double-layered, hollow core with an average diameter of 39 nm, which is separated from the envelope by a 2-3 nm gap. Analysis of the three-dimensional structure suggests that the core is composed of a double-layered chain of nucleocapsid proteins bundled into a hollow ball.
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