Journal
JOURNAL OF GENERAL VIROLOGY
Volume 89, Issue -, Pages 3144-3149Publisher
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.2008/004820-0
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Funding
- Japan Health Science Foundation
- Japanese Ministry of Health, Labor and Welfare [H118-AIDS-W-003]
- Japanese Ministry of Education, Culture, Sports, Science and Technology [18689014, 18659136]
- Grants-in-Aid for Scientific Research [18659136, 18689014] Funding Source: KAKEN
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The matrix domain (MA) of human immunodeficiency virus type 1 Pr55(Gag) is covalently modified with a myristoyl group that mediates efficient viral production. However, the role of myristoylation, particularly in the viral entry process, remains uninvestigated. This study replaced the myristoylation signal of MA with a well-studied phosphatidylinositol 4,5-biphosphate-binding plasma membrane (PM) targeting motif, the phospholipase C-delta 1 pleckstrin homology (PH) domain. PH-Gag-Pol PM targeting and viral production efficiencies were improved compared with Gag-Pol, consistent with the estimated increases in Gag-PM affinity. Both virions were recovered in similar sucrose density-gradient fractions and had similar mature virion morphologies. Importantly, PH-Gag-Pol and Gag-Pol pseudovirions had almost identical infectivity, suggesting a dispensable role for myristoylation in the virus life cycle. PH-Gag-Pol might be useful in separating the myristoylation-dependent processes from the myristoylation-independent processes. This the first report demonstrating infectious pseudovirion production without myristoylated Pr55(Gag)
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