The F protein of Helicoverpa armigera single nucleopolyhedrovirus can be substituted functionally with its homologue from Spodoptera exigua multiple nucleopolyhedrovirus

F proteins of group II nucleopolyhedroviruses (NPVs) are envelope fusion proteins essential for virus entry and egress. An F-null Helicoverpa armigera single nucleocapsid NPV (HearNPV) bacmid, HaBac Delta F, was constructed. This bacmid could not produce infectious budded virus (BV) when transfected into HzAM1 cells, showing that F protein is essential for cell-to-cell transmission of BVs. When HaBac Delta F was pseudotyped with the homologous F protein (HaBac Delta F-HaF, positive control) or with the heterologous F protein from Spodoptera exigua multinucleocapsid NPV (SeMNPV) (HaBac Delta F-SeF), infectious BVs were produced with similar kinetics. In the late phase of infection, the BV titre of HaBac Delta F-SeF virus was about ten times lower than that of HaBac Delta F-HaF virus. Both pseudotyped viruses were able to fuse HzAM1 cells in a similar fashion. The F proteins of both HearNPV and SeMNPV were completely cleaved into F, and F2 in the BVs of vHaBac Delta F-HaF and vHaBac Delta F-SeF, respectively, but the cleavage of SeF in vHaBac Delta F-SeF- infected HzAM1 cells was incomplete, explaining the lower BV titre of vHaBac Delta F-SeF. Polyclonal antisera against HaF(1) and SeF1 specifically neutralized the infection of vHaBac Delta F-HaF and vHaBac Delta F-SeF, respectively. HaF(1) antiserum showed some cross-neutralization with vHaBac Delta F-SeF. These results demonstrate that group II NPV F proteins can be functionally replaced with a homologue of other group II NPVs, suggesting that the interaction of F with other viral or host proteins is not absolutely species-specific.