Journal
JOURNAL OF GENERAL PLANT PATHOLOGY
Volume 80, Issue 6, Pages 475-478Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s10327-014-0549-8
Keywords
Verticillium dahliae; Microsclerotia; Inoculum; Aralia cordata
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Various methods of inoculating plants with Verticillium dahliae have been described; however, so far, there are no effective preparations of its microsclerotia, a major source of infection under natural field conditions. In this study, we developed a simple and effective method to prepare microsclerotial inoculum of V. dahliae using a cellophane medium that effectively promoted microsclerotia formation. In addition, a sieving process allowed the preparation of inoculum exclusively composed of microsclerotia. Our novel method provides a reproducible and stable inoculum for disease induction by V. dahliae under conditions that approximate natural infections.
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