Journal
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
Volume 55, Issue 6, Pages 409-417Publisher
MICROBIOL RES FOUNDATION
DOI: 10.2323/jgam.55.409
Keywords
amino acid transporter; autophagy; Avt6; Saccharomyces cerevisiae; vacuole
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
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Here we examined the significance of Avt6, a vacuolar exporter of glutamate and aspartate suggested by the in vitro membrane vesicle experiment, in vacuolar compartmentalization of amino acids in Saccharomyces cerevisiae cells. Fluorescent microscopic observation of GFP-fused Avt6 revealed it to be exclusively localized to the vacuolar membrane, with the amount of Myc-tagged Avt6 significantly increased under nitrogen starvation. Glutamate uptake by cells was enhanced by deletion of the AVT6 gene, indicating indirect involvement of Avt6 in cellular glutamate accumulation. Differences in acidic amino acid content of both total and vacuolar fractions were insignificant between the parent and avt6 Delta cells when cultured in nutrient-rich conditions. However, in nitrogen-starved conditions, the amount of glutamate and aspartate in the vacuolar fraction was notably increased in the avt6 Delta cells. Avt6 is thus involved in vacuolar amino acid compartmentalization in S. cerevisiae cells, especially under conditions of nitrogen starvation.
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