Journal
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
Volume 29, Issue 10, Pages 1839-1847Publisher
WILEY-BLACKWELL
DOI: 10.1111/jgh.12614
Keywords
biomarkers; iTRAQ; label free; NAFLD; proteomics
Categories
Funding
- Translational Medicine Research Collaboration-a consortium made up of the University of Aberdeen
- Translational Medicine Research Collaboration-a consortium made up of the University of Dundee
- Translational Medicine Research Collaboration-a consortium made up of the University of Edinburgh
- Translational Medicine Research Collaboration-a consortium made up of the University of Glasgow
- NHS Health Board (Grampian)
- NHS Health Board (Tayside)
- NHS Health Board (Lothian)
- NHS Health Board (Greater Glasgow Clyde)
- Scottish Enterprise
- Pfizer
- EPSRC [EP/E49370/1]
- DTI grant New serum Biomarkers for Preclinical and Clinical Drug Safety Assessment
Ask authors/readers for more resources
Background and AimNonalcoholic fatty liver disease (NAFLD) is a common condition affecting up to 25% of the developed world. It is a progressive disease, leading in some to the development of liver cirrhosis. Currently, accurate diagnosis and staging of this condition is only possible with histological examination of a liver biopsy. This gold standard test is neither suitable nor practical for large-scale use as is necessary for a condition as common as NAFLD. The aim of this study is to describe the proteome of human NAFLD using two distinct shotgun proteomic methods, translating the findings into potential biomarkers of NAFLD. MethodsTwo distinct shotgun proteomic techniques (iTRAQ and label free) were used to describe the proteome of NAFLD. Thereafter, candidate biomarkers were selected for validation by ELISA. ResultsOver 550 protein identifications were made in the description of the NAFLD proteome. Several proteins were found to be significantly up/downregulated in nonalcoholic steatohepatitis compared with control, including apolipoprotein E (fold ratio of 1.67), insulin-like growth factor-binding protein 3 (IGFBP3, fold ratio of 1.642), Vitamin D-binding protein (fold ratio of 4.587), and lymphocyte cytosolic protein1 (LCP1, fold ratio of 4.356). ELISA validation of a subset of these proteins confirms the validity of the proteomic studies and suggests possible new biomarkers of NAFLD. ConclusionSerum markers are able to distinguish between the stages of disease in NAFLD as well as detect the grade of fibrosis. Ultimately, noninvasive serum markers may replace liver biopsy in the investigation of patients with suspected NAFLD.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available