Skin, bone and muscle collagen extraction from the trash fish, leather jacket (Odonus niger) and their characterization

Acid soluble (ASC) and pepsin soluble (PSC) collagens were extracted from the skin, bone and muscle of a trash fish, leather jacket (Odonus niger) by three different extraction methods. Method I gave 46-50% yield for ASC, Method II gave 49-58% yield for both ASC and PSC and Method III gave 64-71% yield for PSC. The addition of pepsin had increased the yield by 30-45%. The yields of collagen from skin and bone were higher than muscle. SDS-PAGE pattern revealed that skin and bone collagen as Type I collagen with a typical (alpha 1)2 alpha 2 chains and muscle collagen as Type V collagen with a typical alpha 1 alpha 3 alpha 2 chains. Td values of bone and muscle collagen were high (30-32 A degrees C) compared to skin collagen (27-28 A degrees C). The higher imino acids (190 residues/1,000 residues) were found responsible for the higher Td values. The trash fish, leather jacket can therefore be exploited effectively for collagen as it has got good thermal properties for pharmaceutical and biomedical applications.