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The Challenges of DNA Extraction in Different Assorted Food Matrices: A Review

Journal

JOURNAL OF FOOD SCIENCE
Volume 83, Issue 10, Pages 2409-2414

Publisher

WILEY
DOI: 10.1111/1750-3841.14338

Keywords

deoxyribonucleic acids; extraction methods; food authentication; food matrices; processed foods

Funding

  1. Univ. College of Technology Sarawak [UCTS/RESEARCH/4/2016/06]
  2. Univ. Putra Malaysia [GP-IPS-9625800]

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High-quality DNA extracts are imperative for downstream applications in molecular identification. Most processed food products undergo heat treatments causing DNA degradation, which hampers application of DNA-based techniques for food authentication. Moreover, the presence of inhibitors in processed food products is also problematic, as inhibitors can impede the process of obtaining high qualities and quantities of DNA. Various approaches in DNA extraction and factors in structure and texture of various food matrices affecting DNA extraction are explained in this review.

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