Journal
JOURNAL OF FOOD SCIENCE
Volume 79, Issue 12, Pages M2480-M2484Publisher
WILEY-BLACKWELL
DOI: 10.1111/1750-3841.12594
Keywords
Bacillus cereus; background microflora; comparison; food matrix; selective agars
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Funding
- Korea FDA [10162_Food poisoning_063]
- Mid-career Researcher Program through the Natl Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2012R1A2A2A-01015344]
- Bio-industry Technology Development Program of iPET - Ministry for Food, Agriculture, Forestry, and Fisheries [112137-3]
- Brain Korean 21 (BK21) Project from the Ministry of Education and Human Resources Development
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In this study, we compared the inclusivity, exclusivity, recoverability, and selectivity of the 3 selective agars (mannitol yolk polymyxin B agar [MYPA], polymyxin pyruvate egg yolk mannitol bromothymol blue agar [PEMBA], and Brillance Bacillus cereus agar [BBC agar]) for Bacillus cereus (B. cereus) from pure culture and several food samples. BBC agar showed greater exclusivity and selectivity in pure culture and in foods with high background flora, respectively; however, all the tested media showed similar recoverability (P > 0.05) of B. cereus in pure culture and in most foods. Our results suggest that BBC agar could be useful to enumerate B. cereus from, in particular, food matrixes with high background competing micro flora.
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