Journal
JOURNAL OF FOOD SCIENCE
Volume 76, Issue 6, Pages T138-T143Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1750-3841.2011.02217.x
Keywords
1-aminohydantoin; immunoassay; meat; monoclonal antibody; nanoparticles
Categories
Funding
- Cooperation of Industry, Education and Academy of Guangdong government [2009B090300452]
- Key Project of the National Eleventh-Five Year Research Program of China [2008BAK42B05]
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Due to the potential toxic effects of the nitrofuran family of antibiotics, their use in animals in the food industry has raised health concerns. This study was aimed to develop a lateral flow assay (LFA) based on competitive format for the detection of 1-aminohydantoin (AHD) in meat samples. The assay could be completed in 1 min and detected AHDs derivates (CPAHD) at 3 ng/mL, equivalent to 1.40 ng/mL of AHD, which was much lower than that reported in the literature by similar method. The antibody showed no cross-reactivity with a panel of more than 10 nitrofuran analogs except for nitrofurantoin at a high concentration. The test strip was stable at room temperature for up to 8 wk or at 37 degrees C for 4 wk. Parallel analyses of meat samples with LFA and enzyme-linked immunosorbent assay (ELISA) obtained data in good agreement. This developed gold nanoparticle based LFA had a good specificity, sensitivity, stability, and reliability. It was potentially suitable for on-the-spot large-scale screening of meat samples, and even more other applications.
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