Journal
JOURNAL OF FOOD SCIENCE
Volume 76, Issue 5, Pages C742-C749Publisher
WILEY
DOI: 10.1111/j.1750-3841.2011.02194.x
Keywords
grape; HPLC; multivariate statistical analysis; proanthocyanidin; spectrophotometric assays
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Funding
- Regione Piemonte
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The polymeric proanthocyanidins (PAs) composition of skins and seeds from different Vitis vinifera L. cv. was evaluated, sampling 37 cultivar, preserved in the grapevine collection of Grinzane Cavour (Piedmont, Italy). PAs were quantified using both spectrophotometric (n-butanol and vanillin assay) and chromatographic (high-performance liquid chromatography [HPLC]) methods, and the evaluation of their antioxidant activity was performed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Results of PAs quantification with the different methods were significantly correlated. A strong correlation pointed out between anthocyans and PAs content (particularly regarding HPLC method; r = 0.9359, P = 0), and the antioxidant activity was not dependent on the polymeric PAs content, but negatively correlated with the degree of polymerization (r = -0.6102, P = 1 x 10-4). The hierarchical clustering on principal components analysis allowed grouping the samples in 3 different clusters, observing a direct association between the cluster classification and the anthocyans/PAs content.
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