Journal
JOURNAL OF FOOD SCIENCE
Volume 75, Issue 9, Pages M552-M556Publisher
WILEY
DOI: 10.1111/j.1750-3841.2010.01820.x
Keywords
Box-Behnken design; epsilon-Poly-L-lysine; medium optimization; response surface methodology; Streptomyces violaceusniger
Categories
Funding
- Dept. of Food Science and Nutrition, Huajiachi Campus, Zhejiang Univ., Hangzhou, China
Ask authors/readers for more resources
epsilon-Poly-L-lysine (epsilon-PL) is a novel food biopreservative with broad antimicrobial activity. In the present study, single-factor experiments were applied to screen optimal carbon and nitrogen sources that affect the yield of a epsilon-PL-producing strain, Streptomyces violaceusniger, which was obtained in our laboratory recently. Box-Behnken design and response surface methodology (RSM) were adopted to derive a statistical model for optimizing the composition of the fermentation medium. The results showed that the main factors that affect e-PL production were glucose, carbon source; peptone, organic nitrogen source; and (NH4)(2)SO4, inorganic nitrogen source. The optimum fermentation medium for e-PL production of S. violaceusniger consisted of (g/L) glucose, 62.49; peptone, 5.71; (NH4)(2)SO4, 11.19; MgSO4, 0.5; FeSO4, 0.03; ZnSO4, 0.04; KH2PO4, 1.36; and Na2HPO4 center dot 12H(2)O, 3.58. When cultured in the optimum medium, the e-PL production was an average of 0.349 +/- 0.025 mg/mL, which was 1.75 times higher than the initial medium.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available