Journal
JOURNAL OF FOOD SCIENCE
Volume 73, Issue 2, Pages C60-C66Publisher
WILEY
DOI: 10.1111/j.1750-3841.2007.00619.x
Keywords
acrylamide; antioxidant; bamboo; green tea; kinetics; reduction
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This study investigated the effect of antioxidant of bamboo leaves (AOB) and extract of green tea (EGT) on the formation and kinetics of acrylamide in and equimolar asparagine-glucose model system. The substrates spiked with AOB and EGT were microwave-heated at 180 degrees C and the acrylamide content in final reaction products was quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The results showed that both AOB and EGT could effectively reduce the formation of acrylamide in an asparagine-glucose microwave heating model system and achieved a maximum reduction rate when the addition levels of AOB and EGT were both 10(-6) mg/mL. To describe the kinetic behavior of acrylamide, a simplifed kinetic model was optimized and relative kinetic rate constants were evaluated under isothermal conditions. The results indicated that the reduction effect of AOB and EGT on the acrylamide formation may partly be ascribed to the decrease of the formation rate constant (k(F)) in both AOB and EGT-spiked systems (43.4% and 32.3% of decrease, respectively, P < 0.05). The kinetic parameter k(E), which represents the elimination rate of acrylamide in both AOB and EGT-spiked systems, was not significantly different (6.9% of increase and 10.9% of decrease, respectively, P > 0.05). The results of the kinetic study indicated that addition of AOB and EGT could significantly reduce the formation rate constant (k(F)) of acrylamide, but could not significantly affect the elimination rate constant (k(E)) of acrylamide.
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