Journal
JOURNAL OF FOOD SCIENCE
Volume 73, Issue 6, Pages C458-C463Publisher
WILEY
DOI: 10.1111/j.1750-3841.2008.00839.x
Keywords
Aspergillus awamori; ferulic acid; feruloyl esterase; purification
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Funding
- Chuko Distillers Corp.
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For food industry production processes and other uses, a mold that produces high levels of feruloyl esterase was obtained from laboratory mold collections and other sources. It was Aspergillus awamori G-2 that produces high levels of feruloyl esterase. The feruloyl esterase was purified using ion-exchange chromatography, size-exclusion chromatography, and HPLC chromatography. The enzyme was identified as a monomer protein using size-exclusion chromatography. Its optimum temperature and pH were, respectively, 40 degrees C and pH 5. Its activity was stable at pH 3 to 5. The enzyme was combined with xylan and starch, but it was absorbed by cellulose. The kin of the feruloyl esterase was 0.0019% (0.01 mM). The enzyme showed stable activity at pH 3 and 50 degrees C, making this enzyme useful for food production.
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