Effect of Temperature and Time of Storage on Protein Stability and Anti-Salmonella Activity of Egg White

Hen egg white contains numerous molecules of interest for human health, including antimicrobial proteins Little information is available concerning changes in the antimicrobial activity of egg white during storage; therefore, we analyzed the potential of egg white to inhibit growth of Salmonella enterica serovar Enteritidis following storage at 4, 20. or 37 degrees C for 30 days prior to inoculation Egg white displayed higher anti-Salmonella activity after a few days of storage at 20 and 37 degrees C. The rate of increase in activity was more rapid and pronounced at the higher temperature However, egg white stored at 20 degrees C retained higher antimicrobial activity than that of egg white stored at 4 or 37 degrees C, when the entire storage period is taken in consideration In contrast, storage of egg at 37 degrees C for more than 14 days reduced the bacteriostatic potential of egg white. Statistical analyses revealed a correlation between pH and the antimicrobial activity of egg white Moreover, diminished antimicrobial activity was associated with degradation of ovalbumin and ovotransferrin, as assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and mass spectrometry. However, the fluctuation in anti-Salmonella activity of egg white could not be related to any variation of trypsin-like. chymotrypsin-like. or gelatinolytic activities that potentially account for degradation of antimicrobial egg white proteins