Application of High Pressure Processing To Kill Escherichia coli O157 in Ready-to-Eat Meats

Uncooked ready-to-cat (RTE) meats have previously been identified as vehicles for the transmission of the foodborne pathogen Escherichia coli O157. In this Study. the potential for high pressure processing (HIPP) to kill E. coli O157 ill two RTE meats (Hungarian salami and All Beef salami) was investigated. The RTE meats were inoculated with a five-strain cocktail of E. coli O157, vacuum packed, and then pressure treated at 600 MPa with a hold time of 3 min. Samples were stored at 15 degrees C for 28 days. HPP initially reduced E. Coli numbers on both RTE meats by greater than 4 log, CFU/g. However, with enrichment and immunomagnetic separation we were able to recover E. coli 0 157 from the samples. During! storage. the numbers of E. coli O157 increased on the All Beef samples but remained static on the Hungarian salami. which had a restrictive pH and water activity. Increasing the hold time to 6 or 9 min did not result in additional reduction of E. coli O157. The sensory appeal of the two products was not significantly changed by HPP as determined by a sensory panel (n = 50). Analysis of the reflected light parameters of luminance, green-red, and blue-yellow revealed no significant changes. The results of these experiments indicate that FIPP has potential as a lethal treatment for E. coli O157 oil RTE meats with minimal changes in Consumer appeal.