Journal
JOURNAL OF FOOD PROCESS ENGINEERING
Volume 34, Issue 4, Pages 1199-1219Publisher
WILEY
DOI: 10.1111/j.1745-4530.2009.00523.x
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Phenolic compounds of pomegranate juice, which are responsible for haze formation and browning during storage, could be selectively removed by combined enzyme-ultrafiltration process. The pomegranate juice was treated with Fomes fomentarius laccase at various enzyme concentrations (0.5-5 U/mL), temperatures (20-50C) and times (30-300 min). The effect of these enzymatic treatments on total phenol content and clarity of the juice were evaluated using a central composite design. The optimum treatment conditions were: enzyme concentration 5 U/mL; time 300 min; and temperature 20C. Their application led to a 40% reduction of total phenols but induced a threefold decrease of the juice clarity. This drawback was overcome by ultrafiltration of the laccase-treated juice. The pomegranate juice obtained was clear, stable and characterized by total phenolics = 864 mg/L, clarity (A(650 nm) x 1,000) = 86 and color (A(420 nm) x 1,000) = 236 versus 1,681, 127 and 382, respectively, for untreated juice.
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