Journal
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
Volume 27, Issue 2, Pages 145-150Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2012.05.006
Keywords
Brassica rapa; alpha-Tocopherol; gamma-Tocopherol; Post-harvest; Ascorbate; Dehydroascorbate; Fluorimetric HPLC method; Tocopherol determination; Food analysis; Food composition
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Funding
- Seconda Universita degli Studi di Napoli
- Ministero della Ricerca Scientifica e Tecnologica of Italy [2008S9T3KK_003]
- Regione Campania, Misura 214 azione f2 progetto Agrigenet
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This study describes a reliable method suitable for the quantitative determination of tocopherols with a fast, easy extraction procedure and a rapid, sensitive HPLC fluorescence detection, that results in a better signal-to-noise ratio and lowers the detection limit. The limit of detection of this assay is about 0.06 mg L-1 and the linearity is in a range of 0.06-2 mg L-1. The method has been tested on samples of Brassica rapa L. subsp. sylvestris after harvest as affected by various storage temperatures (4 degrees C, 9 degrees C and 20 degrees C). The fluorescent detection makes it possible to measure tocopherols in these plants even when their concentration is extremely low, as at harvest, without the interference of other metabolites. The increase of tocopherols was followed for 14 days after harvest, and in particular the metabolic profile of a-tocopherol was compared with that of ascorbate, dehydroascorbate and chlorophyll. (C) 2012 Elsevier Inc. All rights reserved.
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