Journal
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
Volume 25, Issue 1, Pages 31-38Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2011.06.002
Keywords
Gas chromatography; Time-of-flight mass spectrometry; beta-Carotene; Tocopherol; Sterol; Policosanol; Rice; Food analysis; Food composition
Categories
Funding
- National Academy of Agricultural Science [PJ0068352011, PJ0068342011, PJ0081842011]
- Rural Development Administration, and Republic of Korea
Ask authors/readers for more resources
An accurate and sensitive method for determining 14 lipophilic compounds, including tocopherols, tocotrienols, sterols and policosanols from rice (Oryza sativa L) grain was developed using gas chromatography (GC) coupled to time-of-flight mass spectrometry (TOFMS). The method is fast, requiring only 13 min for the GC-TOFMS run. During the validation procedure, the method proved to be sufficiently precise and accurate with respect to the degree of endogenous biological variability found in rice samples. The profiles of the 14 lipophilic compounds of pigmented rice, as well as genetically modified (GM) rice to improve beta-carotene, were subjected to principal component analysis (PCA) to evaluate the differences among cultivars. PCA could fully distinguish pigmented rice from nonpigmented rice. However, GM rice could not be separated from its non-transgenic counterpart (Nakdongbyeo). This protocol provides a rapid and feasible method for monitoring the bioactive compounds of rice. (C) 2011 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available