Journal
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
Volume 28, Issue 2, Pages 179-182Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2012.09.002
Keywords
Rubus croceacanthus; Rubus sieboldii; Anthocyanin; Antioxidant activity; ORAC; Berry; Okinawa; Pigments in food; Biodiversity; Wild foods; Food analysis; Food composition
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Anthocyanins from the fruits of Rubus croceacanthus and Rubus sieboldii, wild berries from Okinawa, Japan, were extracted using 80% methanol containing 0.5% acetic acid, and the extracts were separated by a DIAION HP-20 column. The major anthocyanins were purified by preparative HPLC and their structures were determined by NMR and MS spectrometry. Cyanidin-3-O-glucoside and pelargonidin-3-O-glucoside were identified from R. croceacanthus, and their contents were 81.44 +/- 1.01 mg and 170.02 +/- 4.36 mg/100 g fresh weight, respectively. Pelargonidin-3-O-glucoside and pelargonidin-3-O-rutinoside were identified from R. sieboldii, and their contents were 10.04 +/- 0.34 mg and 15.08 +/- 0.48 mg/100 g fresh weight,. respectively. An ORAC assay indicated that the two Okinawan wild berries, especially R. croceacanthus, had potent antioxidant activity compared to raspberry (Rubus idaeus). (C) 2012 Elsevier Inc. All rights reserved.
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