Journal
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
Volume 24, Issue 6, Pages 830-836Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2011.03.007
Keywords
Lutein; Zeaxanthin; RPE cells; Cell viability; Antioxidant enzymes; Reduced glutathione; Intracellular reactive oxygen species; Lipid peroxidation; Food analysis; Food composition
Categories
Ask authors/readers for more resources
Lutein and zeaxanthin are dietary carotenoids which accumulate in human retina, including the retinal pigment epithelial (RPE) cells. Oxidative damage of RPE cells is involved in the pathogenesis age related macular degeneration (AMD). The purpose of this study was to evaluate the potential protective effect of lutein and zeaxanthin against hydrogen peroxide induced oxidative stress in cultured human RPE cells. Human D407 RPE cells were pretreated with lutein and zeaxanthin (10 mu M) for 24 h, and exposed for 1 h to 500 mu M hydrogen peroxide. Cell viability, reactive oxygen species (ROS), the lipid peroxidation and the activity of antioxidant enzymes were determined in basal and oxidative stress conditions. Lutein and zeaxanthin pretreatment protected RPE cells against hydrogen peroxide induced cytoxicity, and inhibited the intracellular ROS generation and lipid peroxidation. Xanthophylls induced a significant increase of superoxide dismutase and glutathione peroxidase activity and of the level of reduced glutathione, but did not significantly modify the catalase activity. These results suggest that lutein and zeaxanthin supplementation can contribute to the prevention of age-related macular degeneration, not only by increasing the macular pigment density, but also by protecting retinal pigment epithelium against oxidative stress. (C) 2011 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available