Journal
JOURNAL OF FLUORESCENCE
Volume 21, Issue 3, Pages 1223-1230Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-010-0801-3
Keywords
Fluorescent dyes; Pluronic; Multiphoton absorption; Bioimaging; Fluorescence microscopy; Lysosomes
Funding
- National Institutes of Health [1 R15 EB008858-01]
- National Science Foundation [CHE-0832622, CHE-0840431]
- US Civilian Research and Development Foundation [UKB2-2923-KV-07]
- Ministry of Education and Science of Ukraine [M/49-2008]
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We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic (R) F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.
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