Journal
JOURNAL OF FLUORESCENCE
Volume 20, Issue 2, Pages 551-556Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-009-0579-3
Keywords
Quantum dots; Tat peptide-conjugated; Micro-PL spectrum; Living cells
Funding
- National Natural Science Foundation of China [10774027, 60638010]
- Shanghai Educational Development Foundation [2008CG03]
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The photoluminescence (PL) spectrum of water-soluble thiol-capped CdTe quantum dots (QDs) conjugated with Tat peptide in solution showed a remarkable redshift as compared to that of unconjugated QDs. After cellular uptake of the Tat-QDs conjugates, the micro-PL spectrum of Tat-QDs in lysosomes showed a spectral blueshift, which was most probably due to the fact that Tat peptide was digested by the enzymes, leaving the Tat-detached QDs in lysosomes. The reasons for the spectral changes have been discussed in detail.
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