Journal
JOURNAL OF FLUORESCENCE
Volume 20, Issue 4, Pages 843-850Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-010-0627-z
Keywords
Curcumin; Cetyltrimethylammonium bromide; Silver nanoparticales; Nucleic acids
Funding
- Natural Science Foundations of China [20575035]
- Shandong Province [Z2008B04]
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It is found that silver nanoparticles (AgNPs) can further enhance the fluorescence intensity of curcumin (CU) - cetyltrimethylammonium bromide (CTAB) - nucleic acids and improve its anti-photobleaching activity. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 2.0 x 10(-8)-1.0 x 10(-6) g mL(-1) for fish sperm DNA (fsDNA), 2.0 x 10(-8)-1.0 x 10(-6) g mL(-1) for calf thymus DNA (ctDNA), 1.0 x 10(-8)-1.0 x 10(-6) g mL(-1) for yeast RNA (yRNA), and their detection limits (S/N=3) are 8.0 ng mL(-1), 10.5 ng mL-(1) and 5.8 ng mL(-1), respectively. This method is used for determining the concentration of DNA in actual sample with satisfactory results. The interaction mechanism is also studied.
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