Journal
JOURNAL OF FLUORESCENCE
Volume 19, Issue 6, Pages 1095-1101Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-009-0509-4
Keywords
Fluorescent nanoparticles; Biological label; Human ovarian carcinoma SKOV-3 cells; Recognition; Detection
Funding
- National Hightech R D program [2007AA06Z402]
- Foundation of Shanghai Municipal Government [08520510400]
- Shanghai Leading Academic Discipline Project [S30406]
- Shanghai Normal University [DZL706]
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In this paper, we report a method for recognizing human ovarian tumor(HOT) cells using fluorescent biological label based on core-shell nanoparticles. The luminescent nanoparticles were synthesized with a water-in-oil(W/O)micromulsion technique. The fluorescent silica core-shell nanoparticles modified with anti-HER2 antibody using bifunctional cross-linker glutaraldedhyde targeted the corresponding tumor antigen in the cell surface of the SKOV3 ovarian cancer cells. The specific immunoreactivity of antibody-nanoparticles with cells was characterized by laser scanning microscopy (LSM) and scanning electron microscope (SEM). The results showed that the method offered potential advantages of sensitivity and simplicity due to high binding efficiency between nanoparticles and cells and provided an alternative method for the detection of HOT.
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