Journal
JOURNAL OF FISH DISEASES
Volume 38, Issue 6, Pages 567-574Publisher
WILEY
DOI: 10.1111/jfd.12268
Keywords
Hsp70; hyperthermia; immune response; Procambarus clarkii; white spot syndrome virus
Funding
- National Natural Science Foundation of China [30901116, 31272455]
- Natural Science Foundation of Zhejiang Province of China [Y3080212]
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White spot disease remains a constant threat to aquaculture worldwide. Hyperthermia has been shown to reduce mortality in white spot syndrome virus (WSSV)-infected shrimps, but the mechanism still remains unclear. In this study, we sought to identify host immune factors that contribute to inhibition of WSSV infection during hyperthermia. In WSSV-infected red swamp crayfish Procambarus clarkii (Girard) cultured at 24 +/- 1 degrees C, transcriptional levels of the heat shock protein 70 (Hsp70) gene showed a modest, 2.2-fold increase in haemocytes following 48h post-infection (hpi). In contrast, in WSSV-infected crayfish cultured at 32 +/- 1 degrees C, Hsp70 gene expression showed a rapid, 19.5-fold induction by 4hpi. This suggests that Hsp70 plays a positive regulatory role in resistance to WSSV infection during hyperthermia. Furthermore, total haemocyte counts (THC) and phenoloxidase (PO) activity were both significantly increased in WSSV-infected crayfish cultured at 32 +/- 1 degrees C by 48hpi. Both may be critical for crayfish survival in the late stages of WSSV infection. Collectively, the up-regulation of host protein Hsp70 expression and increase in THC and PO activity suggest that hyperthermia has immunomodulatory effect that enhanced the resistance of P.clarkii to WSSV infection.
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