Journal
JOURNAL OF FISH BIOLOGY
Volume 78, Issue 4, Pages 1170-1182Publisher
WILEY
DOI: 10.1111/j.1095-8649.2011.02925.x
Keywords
diet selectivity; digestion rate; Lake Erie; PCR; predation; validation
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Funding
- U.S. National Oceanographic and Atmospheric Administration Great Lakes Environmental Research Laboratory (NOAA-GLERL)
- Great Lakes Fishery Commission
- Natural Sciences and Engineering Research Council of Canada
- Ontario Ministry of Natural Resources
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The objective of the study was to validate and apply DNA-based approaches to describe fish diets. Laboratory experiments were performed to determine the number of hours after ingestion that DNA could be reliably isolated from stomach content residues, particularly with small prey fishes (c. 1 cm, < 0 center dot 75 g). Additionally, experiments were conducted at different temperatures to resolve temperature effects on digestion rate and DNA viability. The molecular protocol of cloning and sequencing was then applied to the analysis of stomach contents of wild fishes collected from the western basin of Lake Erie, Canada-U.S.A. The results showed that molecular techniques were more precise than traditional visual inspection and could provide insight into diet preferences for even highly digested prey that have lost all physical characteristics.
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