Journal
JOURNAL OF FISH BIOLOGY
Volume 79, Issue 1, Pages 112-121Publisher
WILEY
DOI: 10.1111/j.1095-8649.2011.02999.x
Keywords
intergenic spacer; Siganus fuscescens; single-strand conformational polymorphism (SSCP); trnH-psbA
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Funding
- PADI Project Aware Foundation
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Traditional visual diet analysis techniques were compared with DNA barcoding in juvenile herbivorous rabbitfish Siganus fuscescens collected in Moreton Bay, Australia, where at least six species of seagrass occur. The intergenic spacer trnH-psbA, suggested as the optimal gene for barcoding angiosperms, was used for the first time to identify the seagrass in fish guts. Four seagrass species and one alga were identified visually from gut contents; however, there was considerable uncertainty in visual identification with 38 of 40 fish having unidentifiable plant fragments in their gut. PCR and single-strand conformational polymorphism (SSCP) were able to discriminate three seagrass families from visually cryptic gut contents. While effective in identifying cryptic gut content to family level, this novel method is likely to be most efficient when paired with visual identification techniques. (C) 2011 The Authors Journal of Fish Biology (C) 2011 The Fisheries Society of the British Isles
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