Journal
JOURNAL OF FISH BIOLOGY
Volume 78, Issue 7, Pages 2074-2079Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1095-8649.2011.02975.x
Keywords
archived tissue; chloroform; formalin; museum specimen; PCR; phenol
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Funding
- European Community [FP6 MIIF-CT-2006-39798]
- National Geographic Society [8541-08]
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A protocol used routinely for rapid ancient DNA extraction was applied to fish tissue archived over 80 years ago. The method proved successful, whereas other extraction protocols failed. Researchers working on DNA from older archived fish samples are encouraged to continue to concentrate their efforts on 'white-eye' specimens, which indicate an alcohol-based fixative and are thus likely to yield viable DNA. (C) 2011 The Authors Journal of Fish Biology (C) 2011 The Fisheries Society of the British Isles
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