Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 215, Issue 10, Pages 2686-2695Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20172064
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Funding
- National Institute of Allergy and Infectious Diseases [R01AI119006, R21AI138020]
- Searle Scholars Program
- Research Council of Norway (program 239757)
- European Union's Seventh Framework Programme for research, technological development, and demonstration under Marie Curie grant agreement [608695]
- CAPES fellowship/PDSE [88881.132337/2016-01]
- Boehringer Ingelheim Fonds fellowship
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI119006, R21AI138020] Funding Source: NIH RePORTER
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We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRI SPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.
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