Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 206, Issue 11, Pages 2483-2496Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20091046
Keywords
-
Categories
Funding
- Solution-Oriented Research for Science and Technology (SORST)
- Ministry of Education, Culture, Sports, Science and Technology
- Global Century COE program of the Ministry of Education, Science, and Culture of Japan
- Grants-in-Aid for Scientific Research [21390062] Funding Source: KAKEN
Ask authors/readers for more resources
Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFR alpha(+)Sca-1(+)CD45(-)TER119(-)) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available