Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 206, Issue 1, Pages 51-59Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20081242
Keywords
-
Categories
Funding
- NCI NIH HHS [R37 CA042471, F32 CA126247-02, F32 CA126247-01, R01 CA042471, F32 CA126247, CA42471] Funding Source: Medline
- NIAID NIH HHS [R01 AI044432, P30 AI073961, AI44432] Funding Source: Medline
Ask authors/readers for more resources
Activation of naive CD8(+) T cells with antigen induces their differentiation into effector cytolytic T lymphocytes (CTLs). CTLs lyse infected or aberrant target cells by exocytosis of lytic granules containing the pore-forming protein perforin and a family of proteases termed granzymes. We show that effector CTL differentiation occurs in two sequential phases in vitro, characterized by early induction of T-bet and late induction of Eomesodermin (Eomes), T-box transcription factors that regulate the early and late phases of interferon (IFN) gamma expression, respectively. In addition, we demonstrate a critical role for the transcription factor Runx3 in CTL differentiation. Runx3 regulates Eomes expression as well as expression of three cardinal markers of the effector CTL program: IFN-gamma, perforin, and granzyme B. Our data point to the existence of an elaborate transcriptional network in which Runx3 initially induces and then cooperates with T-box transcription factors to regulate gene transcription in differentiating CTLs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available