Journal
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
Volume 375, Issue 1-2, Pages 32-40Publisher
ELSEVIER
DOI: 10.1016/j.jembe.2009.05.002
Keywords
Acute hypercapnia; Biomarker; Chronic hypercapnia; Gene expression; Marine fish; NHE-1 gene; Real-time RT-PCR
Categories
Ask authors/readers for more resources
Hypercapnia (increased blood CO2 pressure), due to an increasing of aquatic P-CO2, is a common event under conditions of intensive aquaculture and in fish a complex set of physiological and biochemical alterations are employed to cope with this environmental stress. Many of these adjustments depend to a large extent on changes in the expression of genes that encode for physiologically relevant proteins. In this study we report first on the molecular cloning and characterization of c-Fos and Na+/H+ exchanger-1 (NHE-1) cDNAs in sea bass (Dicentrarchus labrax). Both sequences were deposited in the GenBank with accession no. DQ838581 for c-Fos and EU180587 for NHE-1. We then utilized the real-time PCR technology to monitor dynamic changes in levels of c-Fos and NHE-1 transcripts in response to acute (1 h at 71.42 +/- 14.85 mg/l of free CO2) and chronic (96 h at 47.56 +/- 4.99 mg/l of free CO2) hypercapnia. The c-Fos mRNA abundance was significantly increased in brain in response to both acute and chronic hypercapnia conditions, whereas NHE-1 mRNA abundance was downregulated during acute hypercapnia in gill and kidney, and upregulated in kidney during chronic hypercapnia exposure. These findings suggest that both genes are involved in the response to hypercapnia in sea bass and may be good biomarkers capable of providing useful information about fish welfare. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available